Characterization of Mice with Targeted Deletion of Glycine Receptor Alpha 2
- 1 August 2006
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 26 (15) , 5728-5734
- https://doi.org/10.1128/mcb.00237-06
Abstract
Glycine receptors are ligand-gated chloride channels that mediate inhibitory neurotransmission in the adult nervous system. During development, glycine receptor alpha 2 (GlyRalpha2) is expressed in the retina, in the spinal cord, and throughout the brain. Within the cortex, GlyRalpha2 is expressed in immature cells and these receptors have been shown to be active and excitatory. In the developing retina, inhibition of glycine receptor activity prevents proper rod photoreceptor development. These data suggest that GlyRalpha2, the developmentally expressed glycine receptor, may play an important role in neuronal development. We have generated mice with a targeted deletion of glycine receptor alpha 2 (Glra2). Although these mice lack expression of GlyRalpha2, no gross morphological or molecular alterations were observed in the nervous system. In addition, the cerebral cortex does not appear to require glycine receptor activity for proper development, as Glra2 knockout mice did not show any electrophysiological responses to glycine.Keywords
This publication has 32 references indexed in Scilit:
- Timing and topography of cell genesis in the rat retinaJournal of Comparative Neurology, 2004
- Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator miceThe Journal of Physiology, 2003
- Compound heterozygosity and nonsense mutations in the α1-subunit of the inhibitory glycine receptor in hyperekplexiaHuman Genetics, 2001
- A Nonsense Mutation in the α1 Subunit of the Inhibitory Glycine Receptor Associated with Bovine MyoclonusMolecular and Cellular Neuroscience, 2001
- GABA and glutamate depolarize cortical progenitor cells and inhibit DNA synthesisNeuron, 1995
- Point mutation of glycine receptor α1 subunit in the spasmodic mouse affects agonist responsesFEBS Letters, 1994
- A missense mutation in the gene encoding the α1 subunit of the inhibitory glycine receptor in the spasmodic mouseNature Genetics, 1994
- Functional correlation of fetal and adult forms of glycine receptors with developmental changes in inhibitory synaptic receptor channelsNeuron, 1992
- Isoform-selective deficit of glycine receptors in the mouse mutant spasticNeuron, 1992
- Increased γ‐Aminobutyric Acid Receptor Function in the Cerebral Cortex of Myoclonic Calves with an Hereditary Deficit in Glycine/Strychnine ReceptorsJournal of Neurochemistry, 1990