Protein kinase C stimulation enhances the process formation of adult oligodendrocytes and induces proliferation
- 1 August 1991
- journal article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 29 (4) , 481-489
- https://doi.org/10.1002/jnr.490290408
Abstract
Oligodendrocytes (OL) isolated from adult pig brains regenerate their processes and form a network of fibers after 14–18 days in vitro (DIV). Stimulation of protein kinase C (Pk‐C) by tumour promoters such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) produces at day 7 in vitro a similar network after only 20 hr. H‐7, an inhibitor of Pk‐C, as well as amiloride, which inhibits the subsequent Na+/H+ exchange, reversibly suppress this effect. Activation of the protein kinase A or calmodulin pathway do not result in an increased OL process production. Furthermore, TPA induced proliferation in a subpopulation of OL. We conclude that the stimulation of Pk‐C is of utmost importance for OL regeneration.Keywords
This publication has 34 references indexed in Scilit:
- Radioautographic evidence for slow astrocyte turnover and modest oligodendrocyte production in the corpus callosum of adult mice infused with 3H‐thymidineJournal of Comparative Neurology, 1988
- Phorbol ester enhances morphological differentiation of oligodendrocytes in cultureJournal of Neuroscience Research, 1988
- Phorbol ester stimulates proliferation of astrocytes in primary cultureDevelopmental Brain Research, 1987
- Protein Kinase C‐Activating Tumor Promoters Enhance the Differentiation of Astrocytes in Aggregating Fetal Brain Cell CulturesJournal of Neurochemistry, 1986
- Regulation of cytoplasmic pH by Na+/H+ exchangeTrends in Biochemical Sciences, 1986
- Interaction of tumour promoters with epithelial cells in cultureExperimental Cell Research, 1985
- A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells.The Journal of cell biology, 1984
- Electrophoretic pattern and distribution of cytoskeletal proteins in flat-epitheloid and stellate process-bearing astrocytes in primary cultureNeurochemistry International, 1984
- Phospholipid-sensitive calcium-dependent protein kinase: Inhibition by antipsychotic drugsBiochemical and Biophysical Research Communications, 1981
- A simple embedding technique for monolayer neuronal cultures grown in plastic flasksCells Tissues Organs, 1980