Anti-kinetochore antibodies: use as probes for inactive centromeres.
- 1 March 1985
- journal article
- Vol. 37 (2) , 425-30
Abstract
Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an intensely fluorescent kinetochore (no. 11), the functional centromere, and a less intensely fluorescent kinetochore (no. 9), the inactive centromere. The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.This publication has 17 references indexed in Scilit:
- Autoantibody to centromere (kinetochore) in scleroderma sera.Proceedings of the National Academy of Sciences, 1980
- A tdic(5;15)(p13;p11) chromosome showing variation for constriction in the centromeric regions in a patient with the cri du chat syndromeCytogenetic and Genome Research, 1979
- Induced Robertsonian fusions and tandem translocations in mammalian cell culturesCytogenetic and Genome Research, 1978
- Fusion of two apparently intact human X chromosomesHuman Genetics, 1976
- Preparation and spread of unfixed metaphase chromosomes for immunofluorescence staining of nuclear antigensExperimental Cell Research, 1975
- The possibility of latent centromeres and a proposed nomenclature system for total chromosome and whole arm translocationsCytogenetic and Genome Research, 1975
- New selective Giemsa technique for human chromosomes, Cd stainingNature, 1974
- Apparently isodicentric but functionally monocentric X chromosome in man.1974
- Dicentric and monocentric Robertsonian translocations in manHuman Genetics, 1972
- A RAPID BANDING TECHNIQUE FOR HUMAN CHROMOSOMESThe Lancet, 1971