Alkaline fixation drastically improves the signal of in situ hybridization
- 15 May 2000
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 28 (10) , 46e-46
- https://doi.org/10.1093/nar/28.10.e46
Abstract
In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the influence of the pH of the fixative on the outcome of ISH. Our studies indicate that alkaline formaldehyde dramatically increases the ISH signal with RNA probes. The increase in signal was observed for detection of low as well as for high abundance messages. The sensitivity of the method was increased 5- to 6-fold.This publication has 9 references indexed in Scilit:
- Loss of expression of the candidate tumor suppressor gene ZAC in breast cancer cell lines and primary tumorsOncogene, 1999
- hZAC encodes a zinc finger protein with antiproliferative properties and maps to a chromosomal region frequently lost in cancerProceedings of the National Academy of Sciences, 1998
- Differential mRNA Transport and the Regulation of Protein Synthesis: Selective Sensitivity of Purkinje Cell Dendritic mRNAs to Translational InhibitionMolecular and Cellular Neuroscience, 1996
- In situ hybridization for cytokine mRNA with digoxigenin-labeled riboprobes Sensitivity of detection and double label applicationsJournal of Immunological Methods, 1995
- A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probesHistochemistry and Cell Biology, 1993
- Improved design of riboprobes from pBluescript and related vectors for in situ hybridization.1993
- Poly(A) RNA codistribution with microfilaments: evaluation by in situ hybridization and quantitative digital imaging microscopy.The Journal of cell biology, 1992
- RNA-DNA Hybrids at the Cytological LevelNature, 1969
- FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONSProceedings of the National Academy of Sciences, 1969