Separation of the α Chains of Type I and III Collagens by SDS-Polyacrylamide Gel Electrophoresis1

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Abstract
A method for the separation of type III collagen from type I collagen by SDS-polyacrylamidegel electrophoresis has been developed. This is based on the observation that the presence of 3–4 M urea decreases the mobility of the α1[III] chain to a greater extent than those of the α1[I] and α2 chains, although the α1[I] and α1[III] chains move at the same rate in the absence of urea. An attempt to separate the α1[II] chain of type II collagen from the α1[I] chain was unsuccessful under the experimental conditions employed.