Selective Isolation of Mycoplasma hyosynoviae by Anaerobic Cultivation

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Abstract
Growth of type strains of 23 mycoplasma species and field strains of M. hyorhinis was compared in aerobic and strict anaerobic cultivation. In anaerobic cultivation, the growth of 5 spp. including M. hyorhinis was suppressed; that of the other 4 spp., including M. hyosynoviae, was enhanced. The remaining 14 spp. showed similar growth in the 2 atmospheric conditions. While all anaerobically enhanced species belonged to the arginine-hydrolyzing mycoplasma group, anaerobically suppressed species were all arginine-non-hydrolyzers. In order to inhibit the growth of M. hyorhinis, rapid attainment of strict anaerobic condition was required. In a field survey, the anaerobic cultivation was shown to be effective for selective isolation of M. hyosynoviae from nasal cavities of pigs.