The Blasticidin S Resistance Gene (bsr) Selectable in a Single Copy State in the Bacillus subtilis Chromosome
- 1 June 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 107 (6) , 799-801
- https://doi.org/10.1093/oxfordjournals.jbchem.a123128
Abstract
The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.This publication has 1 reference indexed in Scilit: