The ultrastructural immunolocalization of?-glutamyltranspeptidase in rat lung: Correlation with the histochemical demonstration of enzyme activity

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Abstract
γ-Glutamyltranspeptidase (γ-GT) was localized in slices of rat lung, at the ultrastructural level, by pre-embedding immunogold labelling. Antiserum was raised against the protein purified from rat kidney. The enzyme was found to be concentrated on the lumenal surface of the non-ciliated (‘Clara’) cells of the bronchiolar epithelium and, to a lesser degree, on the surface of type II alveolar pneumocytes. This immunological localization was consistent with the distribution of reaction product, in both slices and resin sections incubated to demonstrate γ-GT activity. γ-GT is probably involved in the utilization of reduced gluthathione (GSH) present in the fluid lining the airway epithelium.

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