High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis of Cerebrospinal Fluid in Patients with Neurological Diseases
- 1 January 1987
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 25 (2) , 61-70
- https://doi.org/10.1515/cclm.1987.25.2.61
Abstract
The protein pattern of cerebrospinal fluid (CSF) of 334 patients with various neurological and systemic diseases were investigated by high resolution two-dimensional electrophoresis (2-DE). 2-DE gels of normal CSF contain proteins which are not detectable in 2-DE gels of serum. Distrubances of the blood-brain or blood-CSF barrier, and degenerative diseases of the brain and malignant diseases produce specific changes on 2-DE gels of the CSF. The appearance of 10 spot areas in the light chain region of 2-DE gels seem to be connected with the diagnosis of multiple sclerosis. The sensitivity and the specificity of these spot areas for the diagnosis of multiple sclerosis are described. Proteins within the ten spot areas are immunoglobulin light chains or substances which cross-react very strongly with light chain antibodies as demonstrated by immunoblotting and immunoabsorption.This publication has 11 references indexed in Scilit:
- Immunoglobulin G, A, and M—Clonal restriction in multiple sclerosis cerebrospinal fluid and serum—Analysis by two-dimensional electrophoresisClinical Immunology and Immunopathology, 1985
- Two‐Dimensional Electrophoresis of Cerebrospinal Fluid and Ventricular Fluid Proteins, Identification of Enriched and Unique Proteins, and Comparison with SerumJournal of Neurochemistry, 1984
- Two‐dimensional electrophoresis of cerebrospinal fluid proteins in multiple sclerosis and various neurological diseasesElectrophoresis, 1984
- Detection of the major non-serum derived proteins of body fluids by immunodeletion and two-dimensional gel electrophoresisElectrophoresis, 1983
- Two-dimensional gel electrophoresis of cerebrospinal fluid proteins.Clinical Chemistry, 1980
- A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gelsAnalytical Biochemistry, 1980
- Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain.Proceedings of the National Academy of Sciences, 1979
- Microheterogeneity of serum transferrin, haptoglobin and α2HS glycoprotein examined by high resolution two-dimensional electrophoresisBiochemical and Biophysical Research Communications, 1979
- High resolution two-dimensional electrophoresis of human plasma proteins.Proceedings of the National Academy of Sciences, 1977