THE DETERMINATION OF OESTROGENS IN BODY FLUIDS

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Abstract
SUMMARY: A micro-method is described for the chromatographic separation of oestrone, oestradiol and oestriol from a phenolic extract of body fluids. The stationary phase is 3·1n aqueous sodium hydroxide held by Celite 535 as the supporting medium. Oestrone and oestradiol are eluted with benzene:petroleum ether, b.p. 60–80° C, 4:1 (v/v), and oestriol with a 17:3-chloroform:butanol mixture. A diagram of the apparatus used is shown. The elution pattern, and data showing how it changes with temperature, are included.