A SENSITIVE GAS CHROMATOGRAPH ASSAY FOR THIOPENTONE IN PLASMA
Open Access
- 1 February 1978
- journal article
- research article
- Published by Elsevier in British Journal of Anaesthesia
- Vol. 50 (2) , 143-145
- https://doi.org/10.1093/bja/50.2.143
Abstract
A sensitive flame ionization gas chromatograph assay method has been developed for thiopentone in human plasma. The extraction procedure minimizes desulphuration of the drug while removing most endogenous substances and is limited by a maximum of 50 ng ml“1 in 2-ml plasma samples. There is linearity throughout the 30.0–5.0 μg ml-1 and 5.0–0.1 μg ml-1 ranges. Extraction of six replicate 2.5-μg ml-1 samples resulted in a coefficient of variation of 3.0%. Similar treatment of six 0.20-μg ml-1 samples afforded a coefficient of variation of 4.6%.Keywords
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