Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.)
- 1 May 1994
- journal article
- Published by Springer Nature in Plant Cell Reports
- Vol. 13 (8) , 468-472
- https://doi.org/10.1007/bf00231969
Abstract
Efficient plant regeneration via somatic embryogenesis has been developed in chickpea cultivar C235. Leaf explants, on MS medium supplemented with 1.25 mg/l 2,4-D and 0.25 mg/l kinetin, yielded somatic embryos with high efficiency during dark incubation. MS medium supplemented with B5 vitamins, 0.125 mg/l IBA and 2 mg/l BAP was found suitable for embryo maturation. The well formed embryos germinated into plantlets on basal B5 medium supplemented with 0.25 mg/l BAP. Further development into healthy plantlets was obtained on basal B5 medium. Hardened plantlets produced normal, fertile plants upon transfer to soil.Keywords
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