Identification and isolation of methionine-cysteine rich proteins in soybean seed
- 1 December 1988
- journal article
- conference paper
- Published by Springer Nature in Plant Foods for Human Nutrition
- Vol. 38 (4) , 287-296
- https://doi.org/10.1007/bf01091726
Abstract
We recently developed a method to identify methionine-containing proteins and quantitate their methionine contents [4]. We applied this method to soybeans and identified relatively methionine-rich proteins (MRP) among the albumins. By acidic methanol extraction of the albumins, we obtained a group of low molecular weight methionine-cysteine rich proteins (MCRP) that analyzed 4.0% methionine and 8.8% cysteine. MCRP made up 1–2% of the total protein in soybeans. Reversed-phase HPLC purification of MCRP yielded a protein peak that exhibited a single major band on denaturing polyacrylamide gel electrophoresis, had a molecular weight of 16kD and contained 6.2% methionine and 18.8% cysteine. We are cloning the gene for this protein. Increasing its level through genetic engineering could increase the methionine-cysteine content of soybeans.This publication has 23 references indexed in Scilit:
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