Comparison Between Dissociation and Inhibition of Association of Dna/Anti-Dna Complexes

Abstract
A study was made of the increases in ionic strength (μ) and in pH that would effect: a) the inhibition of association of antidouble stranded (ds) DNA and dsDNA, and b) the dissociation of antids DNA from dsDNA, with respect to high avidity as well as to low avidity human anti-dsDNA antibodies, using Crithidia luciliae kinetoplasts as a source of dsDNA. The results obtained appear to indicate that the electrostatic component of the antigen-antibody bond does not become stronger with time (for time spans <1 hr).