Loss of Membrane Transport Ability in Leaf Cells and Release of Protein as a Result of Osmotic Shock
- 1 April 1973
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 51 (4) , 620-625
- https://doi.org/10.1104/pp.51.4.620
Abstract
Osmotic shock severely reduces the ability of aged strips of Phaseolus vulgaris leaves to take up α-aminoisobutyric acid, an amino acid analogue which is known to be transported by a specific mechanism. Cold osmotic shock, i.e., transfer from 0.5 m sucrose at 25 C to H2O at 2 C, decreases α-aminoisobutyric acid uptake almost to zero. Substitution of 10−3m ethylenediaminetetraacetate for the sucrose, i.e., treatment which does not involve plasmolysis, produces a similar, but less severe, effect.Keywords
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