The metabolism of [14C]glucose, [14C]fructose and [2−14C]pyruvate after limb ischaemia in the rat

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Abstract
The excretion of C14O2 in the expired air after the intravenous injection of [C14] glucose, [C14]-fructose and [2-C14] pyruvate has been studied in normal rats and rats 1.5 hr. after they had been subjected to a 4 hr. period of bilateral hind-limb ischemia. The disappearance of [C14] glucose from the blood stream after its intravenous injection was also studied in these groups. A mathematical model was developed to account for the distribution and metabolism of [C14] glucose in the controls. This model comprised 2 pools for the body glucose and a 3d pool of bicarbonate CO2 in the path of its excretion as CO2. The anatomical location of these pools has been described as far as possible. The application of the model to the results in the shocked rats showed that the total amount of glucose being metabolized was reduced by 32% and that the amount of glucose being oxidized was decreased by 48%. The rate of excretion of C14O2 from [C14]-fructose was the same as from [C14] glucose in both the controls and the shocked rats, so that there was no evidence for the preferential utilization of fructose by the shocked rat. The oxidation of [2-C14] pyruvate was depressed by 33% in the shocked rats. It was concluded that these changes were not the result of the fall in body temperature after limb ischemia. It is possible that these changes lead to the fall in heat production.