Respiration rate of bacteria as a function of oxygen concentration

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Abstract
A polarographic method of measuring and continuously recording the concn. of O2 in soln. down to the order of 10-9 [image] is descr. This method was used to determine the respiration rate of bacteria as a function of O2 concn. The results obtained show that the respiration rate of bacteria is related to the O2 concn. by the Michaelis-Menten equation. Characteristic Km''s of a number of organisms were detd. The effect of temp. and pH on Km values were investigated. Reasons for differences in Km values of different organisms are discussed and an empirical equation relating Km to the size of the organism is established.