RADIOIMMUNOASSAY FOR ERYTHROPOIETIN

  • 1 January 1979
    • journal article
    • research article
    • Vol. 54  (4) , 885-893
Abstract
Development of a radioimmunoassay (RIA) for erythropoietin was described. Antisera were raised in rabbits with impure human urinary erythropoietin preparation used as immunogen, but with pure human erythropoietin as the labeled antigen in the RIA and as a primary standard. Erythropoietin immunoreactivity was not altered significantly by labeling mode with radioiodine, although biologic activity was lost. With this method, it was possible to detect 2-3 mU [units] of erythropoietin in a 0.1-0.3 ml volume. The method can be used for detection of normal and subnormal serum titers and elevated titers. RIA for erythropoietin does not distinguish between the native (active in vivo) and asialo form (inactive in vivo) and cannot yet be used for routine crude fraction monitoring during purification.

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