Hyaluronan‐binding properties of human serum hemopexin

Abstract
Hemopexin, the heme‐binding serum glycoprotein, exhibits a complex electrophoretic pattern on two‐dimensional immunoelectrophoresis on agarose gels into which hyaluronic acid is incorporated in the first and monospecific anti‐hemopexin in the second dimension. This heterogeneity reflects a range of interactions of hemopexin isoforms with hyaluronic acid. Electrophoretic patterns of individual human sera greatly differ in their contents of hyaluronan‐interacting hemopexin species. Hemopexin itself has no hyaluronidase activity.