FORMATION OF RIBOMONONUCLEOTIDES FROM PURINE AND PYRIMIDINE BASES AND 5′-PHOSPHORIBOSYLPYROPHOSPHATE BY A SALMON MILT EXTRACT
- 30 April 1964
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 42 (5) , 575-581
- https://doi.org/10.1139/o64-070
Abstract
Soluble extracts of immature sockeye salmon milts were prepared by blending them with 0.66 M lithium chloride solution to effect plasmolysis, followed by dilution to 0.10 M concentration with water and removal of the precipitated nucleoprotamine by centrifuging. By means of radioactive tracer technique it was shown that these extracts phosphorylated adenine, guanine, hypoxanthine, thymine, uracil, cytosine, and orotic acid in the presence of α-phosphoribosylpyrophosphate to yield ribomononucleotides.Keywords
This publication has 1 reference indexed in Scilit:
- INCORPORATION OF RADIOACTIVE PURINE AND PYRIMIDINE BASES AND OF THYMIDINE INTO THE DEOXYRIBONUCLEIC ACID OF SALMON MILTSCanadian Journal of Biochemistry, 1964