Design of a glucose minisensor based on streptavidin-glucose oxidase complex coupling with self-assembled biotinylated phospholipid membrane on solid support

1 March 1993

journal article
Published by American Chemical Society (ACS) in Analytical Chemistry

Vol. 65 (6) , 665-668
https://doi.org/10.1021/ac00054a002

Abstract

A simple and fast procedure to prepare a glucose-sensitive minielectrode is presented. It is based on a biotin-modified phospholipid bilayer to which a streptavidin-glucose oxidase complex is coupled. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide at the potential +670 mV. In the case of an air-saturated buffering solution the response to glucose was measured up to 50 mmol.L-1, with a linear portion up to 7 mmol.L-1. The influence of oxygen tension, pH, and temperature as well as possibly interfering substances was investigated. The prospect usage for the measurement of blood and urine was tested.

Keywords

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