Sequence conservation in the RNA polymerase gene of infectious bursal disease viruses
- 1 September 1994
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 134 (3-4) , 441-449
- https://doi.org/10.1007/bf01310582
Abstract
Eleven of thirteen infectious bursal disease viruses (IBDV) were shown to be similar following polymerase chain reaction (PCR) amplification of two regions (293 bp and 651 bp cDNA fragments) in genomic segment B and restriction enzyme analysis of the PCR products. Two IBDV strains differed from the eleven viruses when examined byBstEII digestion of the 293 bp PCR product, but all thirteen viruses were similar using the 651 bp cDNA fragment and digestion withAvaI. Because segment B encodes replicating enzyme(s), the results suggest that its sequences may be highly conserved among IBDV isolates.Keywords
This publication has 25 references indexed in Scilit:
- Observations on polymerase chain reaction amplification of infectious bursal disease virus dsRNAJournal of Virological Methods, 1994
- Comparison of eight different procedures for harvesting avian reoviruses grown in Vero cellsJournal of Virological Methods, 1992
- Characterization of the VPg-dsRNA linkage of infectious pancreatic necrosis virusJournal of General Virology, 1991
- Comparison of neutralizing antigens of recent isolates of infectious bursal disease virusArchiv für die gesamte Virusforschung, 1991
- Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: A putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motifVirology, 1991
- A comparison of the sequences of segment A of four infectious bursal disease virus strains and identification of a variable region in VP2Journal of General Virology, 1990
- Demonstration of enzyme activities required for cap structure formation in infectious bursal disease virus, a member of the birnavirus groupJournal of General Virology, 1990
- Nucleotide sequence analysis of genome segment A of infectious bursal disease virusJournal of General Virology, 1990
- Biochemistry and Immunology of Infectious Bursal Disease VirusJournal of General Virology, 1988
- The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da proteinVirology, 1987