Micropropagation of Granny Smith apple: factors affecting root formationin vitro

Publisher Website
Google Scholar
Abstract
Shoots produced by axillary bud cultures of apple cv Granny Smith, which is difficult to root by standard propagation procedures, were induced to form adventitious roots in vitro. No roots were formed in stationary liquid culture and rooting in agar-based media or with filter paper bridges was low (up to 4%). Up to 80% of cuttings formed roots when grown in continuously agitated liquid culture (half-strength MS) with continuous illumination (10 μE m−2 s−1) and constant temperatures (26° ± 2°C). Exogenous auxin was essential for rooting: IBA at 10 μM and NOA at 10 μM both promoted root formation but 2, 4-D was inhibitory. The optimum sucrose concentration was 1% (w/v). Wounding the cuttings increased root formation. The origin of adventitious roots differed with the auxin applied: with NOA rooting was from cut surfaces and with IBA roots grew both through the epidermis and from cut surfaces.

This publication has 5 references indexed in Scilit: