Surface markers of human gingival fibroblasts in vitro. Characterization and modulation by enzymes and bacterial products

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Abstract
Surface markers of human gingival fibroblasts in vitro were investigated using monoclonal and helerologous unlisera against a range of cell surface antigens, together with resetting techniques to characterize surface receptors for IgG and C., WI-38 fibroblasts and human peripheral blood monocytes were used as control cells. Human gingival fibroblasts exhibited complement receptors and (β2-micro-globulin, as did W1–3S cells. Ten per cent of the human gingival fibroblasts were positive for HLA-DR antigens and additionally exhibited a granulocyte antigen not apparent on WI-3K cells. Monolayers of the gingival fibroblasts were further exposed for short periods to varying concentrations of enzymes (trypsin, collagenase and neuraminidase), bacterial extracts (lipopolysaccharide and lipoteichoic acid) and crude supra- and subgingival plaque sonicates. Surface-marker analysis was then carried out. The most noticeable effects were obtained with Vibrio cholerae neuraminidase which enhanced C3, receptor and surface antigen expression, and supragingival plaque sonicate which depressed the expression of HLA-DR and granulocytc antigens while not affecting β2microglobulin expression. Trypsin reduced antigen expression to a degree, but its effects were mainly on cell adherence.