The chemistry of mould tissue

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Abstract
Different single cell cultures of A. fischeri, grown on synthetic media containing glucose as the sole C source, showed differences in the amt. and nature of the fat, depending on initial conc. of glucose, conc. of NH4NO3, cH, temp., and increased aeration and period of incubation. A high fat content was favored by neutral or slightly alkaline media, a high conc. of glucose and a low conc. of NH4NO3. The % of sterol in the mycelium was increased by the use of a fairly high initial conc. of glucose, 1% NH4NO3 or an initially acid medium containing urea, a higher temp. (37[degree]), and a long period of incubation. The % of lipinphosphorus in the mycelium was increased by the use of low initial conc. of glucose, 1% NH4NO3, and an initially slightly acid medium. During inanition the greater part of the fat (exclusive of unsaponifiable matter) was utilized by the mold. The iodine numbers of the fatty acids were higher when the mold was grown on a low conc. of glucose, on a medium which became strongly acid (e.g., NH4Cl as N source), or at a lower temp. The neutral equivalents of the fatty acids were in all cases very near to 280.