Human granulocyte colony‐stimulating factor receptors in acute myelogenous leukemia

Publisher Website
Google Scholar
Abstract
Human granulocyte colony‐stimulating factor (G‐CSF) receptors on human acute leukemia cells were investigated using human G‐CSF iodolabeled by the lactoperoxidase method. Among various human leukemic cell lines, only cells of myelogenous lineage including HL‐60, THP‐1 and U937 had one type of high‐affinity receptor for G‐CSF, as shown by Scatchard analysis. Fresh leukemia cells from 19 patients with acute myelogenous leukemia (AML) were then studied. Specific receptors for G‐CSF were demonstrated on blast cells in all 19 cases, the mean number of G‐CSF receptors per AML cell ranging from 95 to 1436. G‐CSF receptors on AML cells appeared to be a single affinity type, although some variations were observed. The mean number of G‐CSF receptors on leukemic cells from patients with either FAB M3 or FAB M2 was greater than that of cells from patients with Ml (p < 0.01, p < 0.10, respectively). Moreover, the mean number of receptors for G‐CSF on CD13‐and CD34‐positive AML cells was higher than that on CD13‐negative and CD34‐positive AML cells (p < 0.01), and the mean number of G‐CSF receptors on CD7‐positive AML cells was lower than that for CD7‐negative AML cells (p < 0.10). Since the FAB classification and surface phenotypes reflect maturation stages, our findings indicate that the distribution of G‐CSF receptors, even on AML cells, may be related to the maturation process.