HUMAN-LEUKOCYTE INTERFERON PREPARATION-MEDIATED BLOCK OF GRANULOPOIETIC DIFFERENTIATION INVITRO

  • 1 January 1981
    • journal article
    • research article
    • Vol. 9  (1) , 63-76
Abstract
Human leukocyte-interferon preparation (HLIF) was recently found to block granulopoietic differentiation. Using suspension cultures, it was further demonstrated that HLIF can effectively block granulopoietic differentiation, resulting in an accumulation of granulocyte-macrophage progenitor cells (GM-CFC), cluster-forming cells and the morphologically identifiable myeloid precursors. Using semisolid agar cultures, it was demonstrated that the effect of HLIF on GM-CFC and cluster-forming cells is reversible and that human placental conditioned medium (HPCM), used as a source of colony-stimulating factor (CSF), can effectively counteract the effect of HLIF action on the granulopoietic precursors. The interferon preparation derived from human fibroblasts was less effective in blocking granulopoietic differentiation compared to HLIF, indicating a tissue specificity of interferon action. HLIF may have a regulatory role in the control of granulopoietic proliferation and differentiation.