Kinetic Fluorimetric Method for the Determination of Tobramycin by Stopped-Flow Mixing Methodology
- 1 January 1993
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 26 (1) , 97-107
- https://doi.org/10.1080/00032719308016798
Abstract
The reaction of tobramycin with o-phthaldialdehyde (OPA) in the presence of 2-mercaptoethanol was studied kinetically by using stopped-flow mixing methodology. New data on the kinetics of this reaction were exploited to develop a simple, fast method for tobramycin determination. The linear range (0.05–30.0 μg mL−1), detection limit (0.03 μg mL−1), precision (&RSD 1.92) and selectivity of the proposed kinetic method are quite acceptable. The method was satisfactorily applied to the analysis of pharmaceutical preparations with no pretreatment.Keywords
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