UPTAKE OF TRITIATED LYSINE-VASOPRESSIN BY ISOLATED SECRETORY GRANULES FROM BOVINE NEUROHYPOPHYSES

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Abstract
A subcellular fraction containing neurosecretory granules was obtained by differential centrifugation of homogenized neural lobes of bovine hypophyses. The isolated granules were incubated with tritium labelled lysine-vasopressin and the uptake of the radioactive hormone by the granules was studied. Since enzymes capable of breaking down vasopressin were present in the fraction, it was necessary after the incubation to separate the labelled vasopressin from its radioactive breakdown products. This was done by paper chromatography. The results showed that vasopressin readily reached a higher concentration in the granules than in the surrounding medium. This process was not changed by cold, N2 or metabolic inhibitors. During the incubation radioactivity soon appeared in the proteins of the granules.

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