INVITRO CULTURE CHARACTERISTICS AND REQUIREMENTS FOR EXPRESSION OF RECEPTORS FOR IGM ON HUMAN PERIPHERAL-BLOOD LYMPHOCYTES

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (1) , 222-232
Abstract
The in vitro culture characteristics and requirements for expression of receptors for the Fc portion of Ig[immunoglobulin]M (Fc.mu.) on peripheral blood lymphocytes [PBL] was investigated. Significant numbers of Fc.mu. receptor cells were not detected until after 11 h of in vitro culture. This was not the result of shedding in vivo bound IgM, thus unblocking the Fc.mu. receptors after culturing, but rather an increased number of Fc.mu. receptors on cultured cells, presumably because of de novo synthesis. The Fc.mu. and Fc.gamma. receptors were not mutually exclusive in that a small population of PBL exhibited Fc.mu. and Fc.gamma. receptors and Fc.mu. negative cells may become Fc.mu. positive cells. Fetal bovine serum and human blood group AB serum, which are known to affect differentially various in vitro cell mediated functions, also differentially affect Fc.mu. receptor expression and were capable of modulating the Fc.mu. receptor levels. The diverse and dynamic nature of the Fc.mu. receptor was demonstrated.