Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase
- 1 February 1997
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 179 (4) , 1404-1408
- https://doi.org/10.1128/jb.179.4.1404-1408.1997
Abstract
A highly cross-reactive monoclonal antibody (MAb), 2G10, was found to react in a conserved region of Escherichia coli RNA polymerase sigma70. The epitope was localized to amino acids 470 to 486, which included part of conserved region 3.1. The epitope for MAb 3D3, a MAb which maps close to the 2G10 epitope, was also determined.Keywords
This publication has 30 references indexed in Scilit:
- Crystal Structure of a σ70 Subunit Fragment from E. coli RNA PolymeraseCell, 1996
- Changes in Conserved Region 3 ofEscherichia coliσ70Mediate ppGpp-dependent FunctionsIn VivoJournal of Molecular Biology, 1995
- THE ROLE OF THE SIGMA FACTOR σs (KatF) IN BACTERIAL GLOBAL REGULATIONAnnual Review of Microbiology, 1994
- REGULATION OF THE HEAT-SHOCK RESPONSE IN BACTERIAAnnual Review of Microbiology, 1993
- In vitro functional characterization of overproduced Escherichia coli katF/rpoS gene productBiochemistry, 1993
- Prediction of Protein Secondary Structure at Better than 70% AccuracyJournal of Molecular Biology, 1993
- Sigma subunit of Escherichia coli RNA polymerase loses contacts with the 3′ end of the nascent RNA after synthesis of a tetranucleotideJournal of Molecular Biology, 1991
- Characterization of the Escherichia coli transcription factor .sigma.70: localization of a region involved in the interaction with core RNA polymeraseBiochemistry, 1989
- Structure and function of the .sigma.-70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects on transcriptionBiochemistry, 1988
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970