Storage, Transportation, and Fertility of Undiluted and Diluted Paddlefish Milt
- 1 January 1995
- journal article
- Published by Oxford University Press (OUP) in The Progressive Fish-Culturist
- Vol. 57 (1) , 64-69
- https://doi.org/10.1577/1548-8640(1995)057<0064:stafou>2.3.co;2
Abstract
Milt samples from paddlefish (Polyodon spathula) were collected from specimens obtained from the Missouri River near Chamberland, South Dakota, in 1991 and the Opelika City Reservoir System near Auburn, Alabama, in 1992. Ice-chilled milt samples either were left undiluted or were diluted with one of three chilled extenders, transported overnight on ice, and analyzed for sperm concentration, extracellular pH, electron microscopical characters, sperm motility percentage, and duration of sperm motility. Paddlefish milt had relatively low sperm counts compared with milt of other fish species, averaging 1.8 × 109 spermatozoa/mL. Extracellular pH averaged 8.22. A sperm acrosome was demonstrated by electron microscopy. Dechlorinated tap water and 10% artificial sea water (ASW) activated and sustained sperm motility better than 25% ASW Transportation of milt had no apparent negative effect on fertility. For storage 1–5 d after collection, undiluted milt provided an average fertilization rate (93%) that w...Keywords
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