Humoral and Mucosal IgA Antibody Response to a Recombinant 52-kDa Cysteine-Rich Portion of the Entamoeba histolytica Galactose-Inhibitable Lectin Correlates with Detection of Native 170-kDa Lectin Antigen in Serum of Patients with Amebic Colitis
Open Access
- 1 July 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 174 (1) , 157-162
- https://doi.org/10.1093/infdis/174.1.157
Abstract
Humoral and mucosal IgA responses to a recombinant cysteine-rich portion (designated LC3) of the Entamoeba histolytica galactose-inhibitable lectin's 170-kDa subunit were determined in patients with amebic colitis. All patients had 170-kDa amebic antigen in serum, compared with 1 of SO cyst passers and 1 of 31 controls (P < .01). Seven days after treatment, serum and fecal 170-kDa antigen became undetectable in 12 of the 13 patients (P < .001). Serum anti-LC3 IgA was found in 83.8% of colitis patients, compared with 2% of controls and 12% of asymptomatic cyst passers (P < .001). Salivary and fecal anti-LC3 IgA levels were higher in patients than in cyst passers (P < .001). In conclusion, in amebic colitis, development of humoral and mucosal IgA responses to the recombinant LC3-encoded protein correlates with detection of amebic 170-kDa antigen in serum and feces.Keywords
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