DIAGNOSIS OF BOVINE LEUKOSIS VIRUS-INFECTION BY SOLID-PHASE RADIOIMMUNOASSAY FOR VIRUS-ANTIBODY

  • 1 January 1981
    • journal article
    • research article
    • Vol. 28  (6) , 661-668
Abstract
Solid phase radioimmunoassay (RIA) for detection of anti-bovine leukosis virus (BLV) antibodies in sera of infected animals is described. Viral antigens bound to the surface of polystyrene are able to selectively adsorb the antibodies from serum. Detection of bound antibodies was done quantitatively by 125I-labeled Staphylococcus aureus protein A. Technical details of the assay are reported. The assay could be used as a competitive one as well. Two different methods for detection of anti-BLV antibodies were compared with solid phase RIA. Solid plase RIA is highly sensitive, specific and available for large-scale examination. The assay is recommended for early diagnosis of bovine leukosis.