Cloning of human α1(X) collagen DNA and localization of the COL10A1 gene to the q21–q22 region of human chromosome 6
- 6 May 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 282 (2) , 393-396
- https://doi.org/10.1016/0014-5793(91)80521-4
Abstract
With consensus primers based upon the nucleotide sequence of the chicken α1(X) collagen gene, we have used PCR with human genomic DNA as template to isolate a 289 bp fragment coding for part of the carboxyl non-triple helical domain of the human α1(X) gene. We have demonstrated the presence of the sequence of the PCR clone within the human genome by partial sequence analysis of a 1 kb HindIII genomic DNA fragment that hybridized with the PCR clone. Furthermore, using the PCR clone as a probe for in situ hybridization of human metaphase chromosome spreads, and for Southern analysis of a panel of human—hamster somatic cell hybrid DNAs, we have assigned he locus for the α1(X) gene to the q-21–q22 region of human chromosome 6.Keywords
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