Allele-Specific Receptor-Ligand Interactions in Brassica Self-Incompatibility
- 7 September 2001
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 293 (5536) , 1824-1826
- https://doi.org/10.1126/science.1062509
Abstract
Genetic self-incompatibility in Brassica is determined by alleles of the transmembrane serine-threonine kinase SRK, which functions in the stigma epidermis, and of the cysteine-rich peptide SCR, which functions in pollen. Using tagged versions of SRK and SCR as well as endogenous stigma and pollen proteins, we show that SCR binds the SRK ectodomain and that this binding is allele specific. Thus, SRK and SCR function as a receptor-ligand pair in the recognition of self pollen. Specificity in the self-incompatibility response derives from allele-specific formation of SRK-SCR complexes at the pollen-stigma interface.This publication has 20 references indexed in Scilit:
- A Pollen Coat Protein, SP11/SCR, Determines the PollenS-Specificity in the Self-Incompatibility ofBrassica SpeciesPlant Physiology, 2001
- Self-Incompatibility. Prospects for a Novel Putative Peptide-Signaling MoleculePlant Physiology, 2000
- Cell–cell signaling in the self-incompatibility responseCurrent Opinion in Plant Biology, 2000
- Post-Transcriptional Maturation of the S Receptor Kinase ofBrassicaCorrelates with Co-Expression of theS-Locus Glycoprotein in the Stigmas of TwoBrassicaStrains and in Transgenic Tobacco PlantsPlant Physiology, 2000
- Highly divergent sequences of the pollen self‐incompatibility (S) gene in class‐I S haplotypes of Brassica campestris (syn. rapa) LFEBS Letters, 2000
- The male determinant of self‐incompatibility in Brassica oleracea is located in the pollen coatingThe Plant Journal, 1997
- SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco.Plant Cell, 1996
- Jellyfish green fluorescent protein as a reporter for virus infectionsThe Plant Journal, 1995
- Immunodetection of Protein Glycoforms Encoded by Two Independent Genes of the Self-Incompatibility Multigene Family of BrassicaPlant Physiology, 1990
- Amino-acid sequence of glycoproteins encoded by three alleles of the S locus of Brassica oleraceaNature, 1987