A simple and rapid technique to isolate enriched populations of spermatocytes and spermatids from the immature rat testis
Open Access
- 1 September 1978
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 54 (1) , 67-75
- https://doi.org/10.1530/jrf.0.0540067
Abstract
Summary. A technique was developed to obtain enriched populations of large numbers of primary spermatacytes (70–81%) and of spermatids (75%) from immature rat testes (21–23 and 38 days old respectively) in a simple and rapid fashion. The cells were nearly all viable and membrane preservation was good. The testicular cells were dispersed by a mild mechanical treatment combined with an incubation with purified collagenase, and the cell populations were separated by centrifugation in a discontinuous dextran gradient in a cell culture medium.This publication has 0 references indexed in Scilit: