A Simple Single-step Immunoimmobilisation Method for the Detection of Salmonella in the Presence of Large Numbers of Other Bacteria
Open Access
- 1 February 1975
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 8 (1) , 173-176
- https://doi.org/10.1099/00222615-8-1-173
Abstract
A single-step disk immunoimmobilisation method for the detection of motile salmonellae is described. It combines in a single step the use of the motility of these bacteria in soft agar, their selective growth on Salmonella-Shigella media, and their immobilisation by polyvalent H antiserum. The method is shown to be 10 to 10,000 times more senstitive than a standard method for detection of Salmonella under the experimental conditons used. The time required for detection is shortened to 24-48 h.Keywords
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