Bioconversion of the Anthracycline Analogue Desacetyladriamycin by Recombinant DoxA, a P450-Monooxygenase from Streptomyces sp. Strain C5
- 22 June 2001
- journal article
- letter
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 3 (15) , 2277-2279
- https://doi.org/10.1021/ol015998x
Abstract
[reaction: see text] A recombinant P450-monooxygenase, DoxA, obtained from Streptomyces sp. strain C5, the producer of the anticancer compound daunorubicin, was expressed in S. lividans TK24 and therein used to catalyze the conversion of the anthracycline analogue desacetyladriamycin into the new anthracycline, 10-hydroxydesacetyladriamycin. This work establishes a new function for DoxA and demonstrates the use of a recombinant enzyme to prepare a new anthracycline analogue.Keywords
This publication has 8 references indexed in Scilit:
- The Product of dpsC Confers Starter Unit Fidelity upon the Daunorubicin Polyketide Synthase of Streptomyces sp. Strain C5Metabolic Engineering, 2001
- In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxAJournal of Bacteriology, 1997
- Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5Journal of Bacteriology, 1996
- Wilmsen's reply to DickensAfrican Studies, 1995
- Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthaseGene, 1994
- Significance of anthraquinone formation resulting from the cloning of actinorhodin genes in heterologous streptomycetesMolecular Microbiology, 1992
- Adriamycin, 14‐hydroxydaimomycin, a new antitumor antibiotic from S. Peucetius var. caesiusBiotechnology & Bioengineering, 1969
- Species Interaction in the Fibrinogen–Fibrin ConversionNature, 1964