Pore-forming properties of the major 53-kilodalton surface antigen from the outer sheath of Treponema denticola
- 1 May 1993
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 61 (5) , 1694-9
- https://doi.org/10.1128/iai.61.5.1694-1699.1993
Abstract
A 53-kDa protein from the outer sheath of the oral spirochete Treponema denticola was purified to homogeneity and shown to reconstitute channels in black lipid bilayer model membranes. The channel had a single-channel conductance of 1.8 nS in 0.1 M KCl, making this the largest porin channel observed to date (estimated diameter, 3.4 nm). Electron micrographs of 53-kDa-protein-containing outer sheaths of T. denticola showed a regular hexagonal array of darker staining pits.Keywords
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