Studies of Transfer RNA Tertiary Structure by Singlet-Singlet Energy Transfer
- 1 July 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (7) , 2838-2842
- https://doi.org/10.1073/pnas.71.7.2838
Abstract
Five species of tRNAs bearing two different fluorescent groups were synthesized. These were suitable for intramolecular distance measurements by singlet-singlet energy transfer. The efficiency of energy transfer was determined from the sensitized emission of the energy acceptor. With the assumption that the relative orientation of donor and acceptor is random, the apparent distances between the 5'-end and the 3'-end, the 4-thiouridine and the 3'-end, the pseudouridine and 3'-end, the pseudouridine and the dihydrouridine in Escherichia coli formyl methionine tRNA, and between the 2-thiouridine (in the anticodon) and the 3'-end in E. coli glutamate tRNA were calculated to be 24 A, 38 A, 55 A, 36 A, and >65 A, respectively.Keywords
This publication has 14 references indexed in Scilit:
- Three-dimensional structure of yeast phenylalanine transfer RNA at 3. 0Å resolutionNature, 1974
- On the mRNA induced conformational change of AA-tRNA exposing the T-Ψ-C-G sequence for binding to the 50S ribosomal subunitBiochemical and Biophysical Research Communications, 1974
- Determination of Secondary and Tertiary Structural Features of Transfer RNA Molecules in Solution by Nuclear Magnetic ResonanceProceedings of the National Academy of Sciences, 1973
- Covalent Attachment of a Fluorescent Group to 4-Thiouridine in Transfer RNAThe Journal of Biochemistry, 1973
- Covalent attachment of fluorescent groups to the 5′-end of transfer RNAArchives of Biochemistry and Biophysics, 1973
- Replacement of Y base, dihydrouracil, and 7‐methylguanine in tRNA by artificial odd basesFEBS Letters, 1971
- Studies of Transfer RNA Tertiary Structure by Singlet-Singlet Energy TransferProceedings of the National Academy of Sciences, 1970
- Intramolecular energy transfer in adrenocorticotropinBiochemistry, 1969
- On the Specificity of the Reduction of Transfer Ribonucleic Acids with Sodium BorohydrideEuropean Journal of Biochemistry, 1969
- The interaction of a naphthalene dye with apomyoglobin and apohemoglobinJournal of Molecular Biology, 1965