Nontransmission of deoxynivalenol (vomitoxin) to milk following oral administration to dairy cows

Publisher Website
Google Scholar
Abstract
The absorption of deoxynivalenol (DON; vomitoxin), a trichothecene mycotoxin produced by Fusarium spp., was studied in the dairy cow. Serum and milk DON levels were quantitated following a single oral dose of 920 mg DON to each of 2 lactating cows of similar weight. Maximum blood levels for the 2 animals following DON administration were 200 and 90 ng/ml serum, occurring at 4.7 and 3.5 h, respectively. By 24 h after dosing only trace levels (< 2 ng/ml) were detectable. DON in its conjugated form accounted for 24-46% of the total levels present in serum. Free and conjugated DON were also present in cow''s milk, but only low amounts (< 4 ng/ml) were detected. Detection of DON was carried out utilizing Sep-Pak C18 extraction cartridges for isolation, with additional purification of the sample achieved by passing the extract through a short charcoal/alumina column. The extract was reacted with N-heptafluorobutyrylimidazole prior to quantitation of the resulting DON-tris-heptafluorobutyrate derivative by combined gas chromatography-quadrupole mass spectrometry, using multiple selected ion monitoring. Detection limits were as low as 1 ng/ml (1 ppb).

This publication has 13 references indexed in Scilit: