Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase gene (ILS1)
- 1 February 1989
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 15 (2) , 99-106
- https://doi.org/10.1007/bf00435455
Abstract
The isoleucyl-tRNA synthetase gene (ILS1) from the yeast Saccharomyces cerevisiae was cloned and sequenced. This gene was initially cloned because it cross-hybridizated to what is now presumed to be the isoleucyl-tRNA synthetase gene (cupC) from the protozoan Tetrahymena hhermophila. The ILS1 gene was determined to be 1,072 amino acids in length. A comparison with a recently published sequence of ILS1 1 from another laboratory (Englisch et al. 1987) was made and differences noted. Two promoter elements were detected, one for general amino acid control and one for constitutive transcription. A heat shock protein (hsp70) gene (probably SSA3) was found 237 by upstream from the ILS1 translation start site. The ILS1 amino acid sequence was compared to isoleucyl-tRNA synthetases from other organisms, as well as to valyl-, leucyl- and methionyl-tRNA synthetases. Regions of conservation between these enzymes were found.This publication has 52 references indexed in Scilit:
- A protein required for splicing group I introns in Neurospora mitochondria is mitochondrial tyrosyl-tRNA synthetase or a derivative thereofCell, 1987
- The valyl-tRNA synthetase from Bacillus stearothermophilus has considerable sequence homology with the isoleucyl-tRNA synthetase from Escherichia coliBiochemistry, 1987
- Structure of the Yeast Isoleucyl-tRNA Synthetase Gene (ILS1). DNA-Sequence, Amino-Acid Sequence of Proteolytic Peptides of the Enzyme and Comparison of the Structure to those of other Known Aminoacyl-tRNA SynthetasesBiological Chemistry Hoppe-Seyler, 1987
- E. coli tyrosyl- and methionyl-tRNA synthetases display sequence similarity at the binding site for the 3'-end of tRNABiochemistry, 1986
- A short nucleotide sequence required for regulation of HIS4 by the general control system of yeastCell, 1983
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- Methionyl‐tRNA Synthetase from Escherichia coliEuropean Journal of Biochemistry, 1982
- Extensive regions of homology in front of the two hsp70 heat shock variant genes in Drosophila melanogasterJournal of Molecular Biology, 1981
- Long range control circuits within mitochondria and between nucleus and mitochondriaMolecular Genetics and Genomics, 1980
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977