Preparation of p-Nitrophenyl 2-O-Acetyl-β-d-glucopyranoside and N-Acetyl-β-d-glucosaminidase Activity toward It. (Essential Requirement of 2-Acetamide Group of Substrate for N-Acetyl-β-d-glucosaminidase Hydrolysis)
- 1 January 1973
- journal article
- Published by Oxford University Press (OUP) in Bulletin of the Chemical Society of Japan
- Vol. 46 (1) , 290-291
- https://doi.org/10.1246/bcsj.46.290
Abstract
Paranitrophenyl 2-O-acetyl, and 2,3-di-O-acetyl-β-d-glucopyranoside (VI, VII) were synthesized in order to investigate the effect of the substitution of the amide-nitrogen of the substrate by oxygen on the N-acetyl-β-d-glucosaminidase hydrolysis. Paramethoxybenzylidene was used as an O-blocking group for the preparation, and it was found that this blocking group could be easilly removed without the destruction of p-nitrophenyl glycoside. The evidence that VI could not be hydrolysed with this enzyme indicated the essential requirement of the amide-nitrogen of the substrate for the enzyme action.Keywords
This publication has 2 references indexed in Scilit:
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- Oligonucleotidic compounds. V. 2',3'-O-Alkylidene derivatives of ribonucleosidesCollection of Czechoslovak Chemical Communications, 1963