FLOW - Injection Determination of Traces of Sulfide by the Brilliant Green - Sulfide Reaction with Spectrophotometry Detection

Publisher Website
Google Scholar
Abstract
A flow injection system is proposed for the rapid and sensitive determination of trace amounts of sulfide based on the addition reaction of sulfide with Brilliant Green at pH 7 and 25 C°. The effect of important parameters, such as reagent concentration, pH, reagent flow rate, sample volume, temperature and length of the reaction coil are reported. Sulfide in the range of 40 - 2000 ng·ml−1 can be determined at a rate of 40 ± 5 samples per hour. The limit of detection was obtained as 2.0 ng of sulfide. The relative standard deviation for the determination of 100 ng·ml−1 of sulfide is 0.8%. A system is proposed for elimination of potential interferences. The method was applied to the determination of sulfide in synthetic samples and in spring water.