Characterization of proopiomelanocortin mRNA detected by in situ hybridization
Open Access
- 1 January 1986
- journal article
- research article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 6 (1) , 38-42
- https://doi.org/10.1523/jneurosci.06-01-00038.1986
Abstract
In situ hybridization is a method for detecting specific nucleotide sequences by using a labeled complementary nucleic acid probe. We have characterized in situ hybridization in rat pituitary using a cDNA probe directed against a portion of proopiomelanocortin (POMC) mRNA to show its specificity in tissue sections. This characterization includes the size of the target mRNA from tissue processed for in situ hybridization, the dissociation pattern of the cDNA:mRNA hybrid, and quantitation of changes in POMC mRNA levels in the rat pituitary intermediate lobe detected both by in situ hybridization and “dot blot” techniques.This publication has 16 references indexed in Scilit:
- Radioautographic localization of prolactin messenger RNA on histological sections by in situ hybridizationBrain Research, 1981
- HYBRIDIZATION HISTOCHEMISTRY : USE OF RECOMBINANT DNA AS A “HOMING PROBE” FOR TISSUE LOCALIZATION OF SPECIFIC mRNA POPULATIONS.Endocrinology, 1981
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Corticotropin and beta-endorphin: construction and analysis of recombinant DNA complementary to mRNA for the common precursor.Proceedings of the National Academy of Sciences, 1979
- Detection of viral sequences of low reiteration frequency by in situ hybridization.Proceedings of the National Academy of Sciences, 1978
- Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.Journal of Histochemistry & Cytochemistry, 1978
- Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesisBiochemistry, 1978
- Effects of Hypothalamic Lesions and Drugs Interfering with Dopaminergic Transmission on Pituitary MSH Content of RatsNeuroendocrinology, 1978
- Detection of myosin heavy chain mRNA during myogenesis in tissue culture by in vitro and in situ hybridizationCell, 1977