Glycopeptides from epithelial cell mutants: Temperature sensitive for the transformation phenotype

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Abstract
Fucose-labelled glycopeptides obtained from the cell surfaces of normal and transformed epithelial cells were compared by co-chromatography on Sephadex G-5O. The material obtained from rat epithelial cells transformed in vitro or from hepatoma cells in culture elutes earlier than the fucosecontaining glycopeptides obtained from normal rat epithelial cells. A mutant (TS 223) of a transformed epithelial cell that is temperaturesensitive for maintenance of the transformed phenotype, varies in its Sephadex G-50 profile of cell surface glycopeptides when grown at the permissive (34° C) or the non-permissive temperature (40° C). When grown and labelled at 36° C the gel filtration profile of the glycopeptides resembles that of transformed cells. At 40° C there is an enrichment of later eluting glycopeptides. These differences are more striking in confluent-phase cultures than in log-phase cultures. The differences are reversible following upward or downward shifts in growth temperature although there appears to be a lag of at least 6 h before the alteration can be demonstrated by these procedures.