The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions
- 1 July 1998
- journal article
- review article
- Published by Springer Nature in Nature Biotechnology
- Vol. 16 (7) , 633-638
- https://doi.org/10.1038/nbt0798-633
Abstract
The analysis of macromolecular interactions is an essential element of biomedical research. Flow cytometry is uniquely capable of making sensitive and quantitative measurements of molecular interactions. These measurements can be made in real time with subsecond kinetic resolution using purified biomolecules or living cells. Combined with automated sample handling, these features make flow cytometry a versatile and robust technology for the analysis of molecular interactions.Keywords
This publication has 20 references indexed in Scilit:
- Applications of the green fluorescent protein in cell biology and biotechnologyNature Biotechnology, 1997
- [19] Fluorescence approaches to study of protein-nucleic acid complexationPublished by Elsevier ,1997
- [11] Fluorescence methods for studying equilibrium macromolecule-ligand interactionsPublished by Elsevier ,1997
- Single-Molecule Fluorescence Analysis in SolutionApplied Spectroscopy, 1996
- A rapid mix flow cytometer with subsecond kinetic resolutionCytometry, 1995
- Flow cytometric detection of human immunodeficiency virus type 1 proviral DNA by the polymerase chain reaction incorporating digoxigenin‐ or fluorescein‐labeled dUTPCytometry, 1995
- Model system evaluating fluorescein‐labeled microbeads as internal standards to calibrate fluorescence intensity on flow cytometersCytometry, 1989
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- DNP‐phycobiliproteins, fluorescent antigens to study dynamic properties of antigen‐IgE‐receptor complexes on RBL‐2H3 rat mast cellsCytometry, 1987
- Time: A New Parameter for Kinetic Measurements in Flow CytometryScience, 1980