RNA-Binding Proteins of Rabbit Reticulocytes. Isolation and Electrophoretic Characteristics

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Abstract
A complete set of RNA-binding proteins was isolated from the ribosome-free extract of rabbit reticulocytes using the method of affinity chromatography on RNA covalently coupled with Sepharose. The purity of the isolated proteins was no less than 90%. These proteins comprised about 1% of the total protein of the extract and included the main polypeptide chains of 3 sizes, with MW of about 95,000, 49,000 and 36,000, as well as numerous minor components. An analogous set of proteins was observed as a result of chromatography of the extract on a column of poly(U) covalently coupled with Sepharose. The protein with the 49,000 MW had the highest affinity for RNA.