Characterization of One Neutral and Two Acidic Glycoasparagines Isolated from the Urine of Patients with Aspartylglycosylaminuria (AGU)1
- 1 August 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 80 (2) , 195-201
- https://doi.org/10.1093/oxfordjournals.jbchem.a131264
Abstract
One neutral and two acidic glycoasparagines were isolated from the urine of patients with aspartylglycosylaminuria (AGU). The neutral one was identified as β-Gal-(1↑4)-β-GICNAc-Asn. The acidic ones were composed of 1 mole of sialic acid and 2 moles each of galactose and N-acetylglucosamine, attached to asparagine, and were isomeric with respect to the position of sialic acid attachment since they produced the same glycoasparagine on incubation with the neuraminidase [EC 3.2.1.18] from Clostridium perfringens. The structure of the resulting sialic acid-free glycoasparagine was determined to be β-Gal-β-GIcNAc-β-Gal-(1↑4)-B-GlcNAc-Asn based mainly on the results of sequential enzymatic degradations.Keywords
This publication has 4 references indexed in Scilit:
- The Thiobarbituric Acid Assay of Sialic AcidsJournal of Biological Chemistry, 1959
- A MODIFIED COLORIMETRIC METHOD FOR THE ESTIMATION OF N-ACETYLAMINO SUGARSJournal of Biological Chemistry, 1955
- A SUBMICRODETERMINATION OF GLUCOSEJournal of Biological Chemistry, 1949
- Separation of serum albumin into two fractionsBiochemical Journal, 1937